Psin-ef2 vector
WebFeb 3, 2024 · Lentiviral vector construction, virus infection and the establishment of stable cell line. The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral … WebJan 8, 2024 · The pSin-EF2-puro-ARNTL, pSin-EF2-puro-vector and psin-EF2-puro-CDK5 plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China), and the psin-EF2-puro-vector plasmid served as a control. To generate stable cell lines, lentivirus packing expression plasmids were co-transfected into 293 T cells for 48 h. Then, SUNE1 …
Psin-ef2 vector
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WebJul 6, 2024 · Although pSIN-EF2-(Pou4f3 or GFI1)-Pur and pLV-ATOH1-IRES-eGFP belong to the third-generation of lentiviral transfer vectors [36,37], pSIN-EF2-Pou4f3-Pur and pSIN … WebB: Schematic construction of vectors encoding soluble scFv C1 and scFv C1-N1N2. The amber stop codon between the scFv and gene III in pHEN 2 was removed by mutagenesis …
WebNov 17, 2024 · GFP-human Med15 and TagRFP-human DYRK3 fusion protein were subcloned into the lentiviral expression vector pSin-EF2 by ClonExpress II One Step Cloning Kit (Vazyme Biotech, C112). Primers used to clone lentiviral vectors are provided in Additional file 6: Table S3. The human embryonic kidney 293T cell line was used as a host … WebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal …
WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty pSin-EF2 vector was then co-transfected ... Web伟通生物现成基因表达载体库开放供应伟通生物根据基因功能研究需要,制备和积累近千个基因表达载体。其中大基因的序列都经过测序验证,与ncbi上的注册序列保持一致。这些载体既可以直接作为基因功能研究使用,也可以作为新构建载体的模板。伟通将部分常用的,不涉及第三方知识产权的表达 ...
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WebJun 1, 2024 · The lentiviral expressing vector encoding human Oct4 (pSin-EF2-Oct4-Pur, Addgene plasmid 16579) was obtained from Addgene (Cambridge, MA) . The lentiviral control vector pSin-null was derived from pSin-EF2-Oct4-Pur as previously described . For knockdown experiments, pLKO.1-puro-based lentiviral vectors, including stem-loop … how to send a prisoner a packageWebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector … how to send apple pay money to bank accountWebApr 20, 2012 · Fig. 7 outlines the vector (pSin-EF2-TK-Puro), with TK driven by the EF1α promoter. hES H9 cells were treated with BMP4 , allowing for trophoblast differentiation. Cells were infected with either the SSEA4 or CD24 antibody conjugated m 168 pseudotyped virus bearing the pSin-EF2-TK-Puro lentiviral vector, five days post BMP4 addition and ... how to send a prescription in epicWebApr 27, 2024 · The DAB2IP expression construct was generated by subcloning PCR-amplified full-length human DAB2IP complementary DNA into the pSin-EF2-puro lentiviral vector (Addgene, beijing, China) by EcoRI and NheI. pSin-EF2-puro-DAB2IP or the empty lentiviral vector and 2 packaging plasmids pM2.G and psPAX2 (Addgene, beijing, China) … how to send appointment in outlookWebJan 7, 2024 · Product Name : pSin-EF2-Sox2-Pur article : bacterial resistance : Ampicillin cloning : growth notes : The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. origin : 37 pi : plasmid copy : SINF-EF-G (from Dr. Robert Hawley, George Washington University) was modified to make the lentiviral backbone. how to send a private outlook meeting inviteWebAddgene inc psin ef2 modulation vector Psin Ef2 Modulation Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. … how to send a pretimed emailWebAug 14, 2015 · zt085 腺病毒载体系统 pAd/BLOCK-iT-DEST RNAi Gateway Vector Invitrogen ... zt1273 pSin-EF2-puro-OCT4 . zt1274 pSIREN-retroQ . zt1275 psiSTRIKE . zt1276 pST1347-cas9-D10A . zt1277 PT/neo(2200) zt1278 PT2/GFP(2000) zt1279 PT2/mCherry(321) zt1280 PT2/RMCE-puDTK(1500) ... how to send a premiere pro project