WebCembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 expression on tobacco resistance to … WebApr 13, 2024 · The three primer made use of in the qPCR was the mRQ three primer supplied using the kit. Reverse transcription of mRNA was performed using the PrimeScriptTM RT Master Mix (TaKaRa), while RT-qPCR was performed working with the One particular Step TB GreenPrimeScriptTM RT-qPCR Kit II (TaKaRa) and normalized to -actin.
PrimeScript RT Reagent Kit (Perfect Real Time
WebApr 11, 2024 · Branching is an important agronomic and economic trait in cut chrysanthemums. The axillary meristem (AM) formation of the axillary buds of cut chrysanthemums has a decisive role in its branching characteristics. However, little is known about the regulation mechanism of axillary meristem formation in chrysanthemums at the … WebHigh Capacity cDNA Reverse Transcription Kit USER GUIDE For 200 and 1000 reaction kits Catalog Numbers 4368813, 4368814, 4374966, 4374967 Publication Number MAN0017977 Revision A.0. Manufacturer: Thermo Fisher Scientific Baltics UAB V.A. Graiciuno 8, LT-02241 Vilnius, Lithuania david harland showjumper
Primescripttm Reverse Transcription Rt Reagent Kit TaKaRa Bioz
WebAtopic dermatitis (AD) is a chronic and inflammatory skin disease that can place a significant burden on quality of life for patients. AD most frequently appears under the age of six and although its prevalence is increasing worldwide, therapeutic treatment options are limited. Chlorella vulgaris (CV) is a species of the freshwater green algae genus chlorella, … WebDec 30, 2024 · Total RNA used for qRT-PCR was extracted using HiPure Plant RNA Mini Kit (R4151-02, Mage). Then the RNA was reverse transcribed to the first-strand cDNA using the Reverse Transcription Kit (PrimeScriptTM RT Master Mix, Takara). The qRT-PCR was performed with a CFX96 (Bio-rad, USA) and the SYBR qPCR Master Mix kit (Vazyme). WebSerum response factor (SRF) is a transcription factor which is critical in myocyte differentiation and growth. In this study, we established a mouse COPD model induced by cigarette smoking ... according to the manufacturer’s protocol. cDNA was then prepared from 1,000 ng total RNA using a PrimeScript reverse transcription (RT) reagent kit ... gas powered airsoft sniper rifles