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Mcherry-egfp-lc3b

Web27 aug. 2024 · Additionally, a tandem mCherry-EGFP-LC3B construct was used to further investigate the impaired autophagic flux. In cells treated with Earle’s Balanced Salt … WebpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染细胞后能够在靶细胞中高效 …

维真 mCherry-GFP-LC3B 自噬双标腺相关病毒产品说明 - Bio-Equip

WebThe mCherry-EGFP-LC3B-expressing cells were seeded in 24-well flat-bottom plates at a density of 120,000 cells/well and on the next day treated as indicated for 24 h. The FACS experiments were performed on a FACS BD LSR II (BD Biosciences, San Jose, CA, USA) using BD FACSDiva software. WebTHP1-Difluo™ hLC3 reporter cells are designed to monitor the autophagic flux by means of a fluorescent-labeled LC3B protein. These cells, derived from the THP-1 human … batman messias https://amdkprestige.com

GFP::LC3B vector to visualize autophagosome formation in real-time

WebMonitoring autophagic flux is important for the analysis of autophagy. Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux … Web24 aug. 2024 · To further validate that ATL2 regulates only ER-phagy and not general autophagy, we used cells stably expressing mCherry-eGFP-LC3B to measure general autophagy flux by flow cytometry. We found that general autophagy is inhibited only in cells lacking ATG10 but not lacking ATL2 ( Fig. 2 H ). Web12 aug. 2024 · mCherry (红光)-eGFP (绿光)-LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。 它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的 … test na poziom stresu

Autophagy restricts Mycobacterium tuberculosis during acute …

Category:Autophagy deficiency exacerbates iron overload induced reactive …

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Mcherry-egfp-lc3b

Lenti-EGFP-LC3B (10^9TU/ml)(EGFP-LC3B慢病毒)(C3003-100μl)

WebVector type Mammalian Expression, Retroviral Selectable markers Puromycin Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C … WebLenti-mCherry-EGFP-LC3B (C3002)和Lenti-EGFP-LC3B (C3003)适合用于一些腺病毒较难感染或者需要筛选稳定细胞株的情况；Ad-mCherry-GFP-LC3B (C3011)和AdPlus …

Mcherry-egfp-lc3b

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Web8 feb. 2024 · C, A375P cells expressing the mCherry-eGFP-LC3B reporter were treated with HCQ, Lys05, or DC661 (0–100 μmol/L, 1 hour) and analyzed by microscopy. D, A375P cells were treated with HCQ, Lys05, or DC661 (3 μmol/L, 6 hours) and stained with LysoSensor and imaged with fluorescent microscopy. Quantitation is shown to the right. … Web7 jan. 2024 · home > Addgene > pDEST-CMV mCherry-GFP-LC3B WT product summary. company name : Addgene. product type : cDNA. product name : pDEST-CMV mCherry …

WebpCMV-mCherry-GFP-LC3B: 1μg: 888.00元: 加入购物车: D2707-100μg: pCMV-N-EGFP (绿色荧光蛋白) 100μg: 1169.00 ... Web南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出

Web2 jun. 2011 · Hundeshagen and colleagues used GFP-LC3B in an initial screen by flow cytometry to quantify autophagosome formation in response to a library of 1,120 Food … http://www.geneppl.com/x3.php?id=6

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Web17 jun. 2024 · 由于细胞自噬在生理和病理过程中都有重要作用，自噬已经成为细胞生物学领域的一个 新的研究热点。. Ad-mCherry-GFP-LC3B是碧云天自行研发的重组腺病毒， … test na raka jelita grubegobatman metalhttp://www.wzbio.com.cn/newsDetail.php?id=33&pid=34&cid=35 batman metal car decalWebThe LC3B protein plays a critical role in autophagy. Normally, this protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3B … test na poziom jezykaWeb27 apr. 2024 · LC3B is one of the most commonly used markers to measure autophagy. It is present at autophagic vesicles very early on and stays attached even when the autophagosome reaches the lysosome. To... test na psani na klavesniciWebQuantification of eGFP-mCherry-LC3B puncta G (n = 3). eGFP fluorescence was measured by flow cytometry in L6 cells stably expressing tandem fluorescent eGFP-mCherry-LC3B treated with with 250 μM FeCl 3 for 1, 2, 4 or 24 h after saponin permeabilization and cytoplasmic washout of the cells. eGFP dMFIs were quantified by subtracting the MFI of … test na prijimacky na stredne skolyhttp://www.bioon.com.cn/sub/showarticle.asp?sub_id=0&newsid=98676 test na raka prostaty