G3bp transcriptomics
WebFunction. ATP- and magnesium-dependent helicase that plays an essential role in innate immunity ( PubMed: 30510222 ). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA ( PubMed ... WebOct 11, 2024 · Background: Microvesicles (MVs) expressing the type 1 interferon (IFN)-inducible protein galectin-3 binding protein (G3BP) may play a pathogenic role in systemic lupus erythematosus (SLE). Co-expression of double-stranded DNA (dsDNA) on such MVs may render them immunogenic and targets for anti-dsDNA antibodies. Little is known …
G3bp transcriptomics
Did you know?
WebJun 25, 2024 · Fig. 2 Ubiquitination of the NTF2L domain of G3BP1 is required for the disassembly of stress granules. ( A) Immunoblot of transfected HEK293T cell extracts captured with antibody to GFP after exposure to no stress, heat shock for 1 hour, or 37°C recovery for 30 min. ( B) Dimeric structure of G3BP1 NTF2L domain. WebAug 17, 2024 · Guillen-Boixet, J. et al. RNA-Induced conformational switching and clustering of G3BP drive stress granule assembly by condensation. Cell 181 , 346–361 (2024). …
WebHere we show that G3BP is essential for SG condensation initiated by p-eIF2α or eIF4A inhibition, but dispensable for SGs induced by hyperosmolarity or severe heat shock. G3BP-mediated SG assembly is regulated by mutually exclusive binding of Caprin1 and USP10, and requires its RGG region that allows interactions with 40S subunits. WebJul 1, 2016 · N) of the G3BP-binding region of SFV nsP3 . The structure demonstrated that FGDF bound to the same hydrophobic pocket as the nucleoporin-derived FxFG motif. Interestingly, single FGDF motifs exist in the herpes simplex virus (HSV)-1-infected cell protein (ICP) 8, the cellular G3BP-interacting protein USP10 and probably other proteins …
WebDec 22, 2024 · Sequence characteristics of G3BP proteins. (a) Schematic representation of G3BP1 and G3BP2 transcripts. G3BP regulating sequences are indicated (red: DDX5-binding site, yellow: TAR DNA-binding protein 43-binding site). ... Inspection of transcriptomics data from several brain cell subtypes such as astrocytes, …
WebMar 28, 2016 · G3BP interacts with 40S ribosomal subunits through its RGG motif, which is also required for G3BP-mediated SG formation. We propose that G3BP mediates the …
WebJun 14, 2024 · G3BP-1 and -2 (hereafter referred to as G3BP) are multifunctional RNA-binding proteins involved in stress granule (SG) assembly. Viruses from diverse families target G3BP for recruitment to replication or transcription complexes in order to block SG assembly but also to acquire pro-viral effects via other unknown functions of G3BP. dutch oven ribeye recipeWebApr 16, 2024 · These results also highlight G3BP as an important node within the network of interactions that underlie SG assembly and as an essential factor in the context of arsenite stress. However, the … crysal liquid thermometerWebG3BP1 (G3BP, HDH-VIII) protein expression summary. We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all … dutch oven ribeye steakWebFeb 4, 2013 · Transcriptomics data indicate that G3BP1 is expressed at relatively low levels in neurons (GTEx) and this could suggest that it is subject to very fine/tight … dutch oven rib roast recipeWebApr 16, 2024 · G3BP Proteins Have the Highest Centrality within the Core SG Network To identify genes essential for SG assembly, we conducted a genome-wide RNAi screen in which we targeted 21,121 genes with pools of four different small interfering RNAs (siRNAs) per gene (84,484 total siRNAs) in a U2OS cell line expressing a G3BP1-GFP reporter ( … dutch oven ribeye roastWebG3BP interaction is required for high localized translational activity around viral replica-tion complexes. We find the RGG domain of G3BP to be essential for the recruitment of the host translation machinery. In cells expressing mutant G3BP lacking the RGG domain, SFV replication was attenuated, but detectable, while CHIKV was essentially non ... crysalis pro downloadWebApr 16, 2024 · Stressed cells shut down translation, release mRNA molecules from polysomes, and form stress granules (SGs) via a network of interactions that involve G3BP. Here we focus on the mechanistic underpinnings of SG assembly. We show that, under non-stress conditions, G3BP adopts a compact auto-inhibited … dutch oven rib roast